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Kinetic Characterization of Single Strand Break Ligation in Duplex DNA by T4 DNA Ligase
T4 DNA ligase catalyzes phosphodiester bond formation between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA in three steps: 1) enzyme-adenylylate formation by reaction with ATP; 2) adenylyl transfer to a 5′-phosphorylated polynucleotide to generate adenylylated DNA; and 3) phosphodie...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3243518/ https://ncbi.nlm.nih.gov/pubmed/22027837 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.284992 |
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