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Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation
Padlock probes are single-stranded DNA molecules that are circularized upon hybridization to their target sequence by a DNA ligase. In the following, the circulated padlock probes are amplified and detected with fluorescently labeled probes complementary to the amplification product. The hallmark of...
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| Publicado no: | RNA |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5238799/ https://ncbi.nlm.nih.gov/pubmed/27879431 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.057836.116 |
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