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Whole mount in situ hybridization detection of mRNAs using short LNA containing DNA oligonucleotide probes
In situ hybridization is widely used to visualize transcribed sequences in embryos, tissues, and cells. For whole mount detection of mRNAs in embryos, hybridization with an antisense RNA probe is followed by visual or fluorescence detection of target mRNAs. A limitation of this approach is that a cD...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Cold Spring Harbor Laboratory Press
2010
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2822927/ https://ncbi.nlm.nih.gov/pubmed/20086052 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1775610 |
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