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Identification and remediation of biases in the activity of RNA ligases in small-RNA deep sequencing
Deep sequencing of small RNAs (sRNA-seq) is now the gold standard for small RNA profiling and discovery. Biases in sRNA-seq have been reported, but their etiology remains unidentified. Through a comprehensive series of sRNA-seq experiments, we establish that the predominant cause of the bias is the...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3241666/ https://ncbi.nlm.nih.gov/pubmed/21890899 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr693 |
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