RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries

Sequencing of small RNA cDNA libraries is an important tool for the discovery of new RNAs and the analysis of their mutational status as well as expression changes across samples. It requires multiple enzyme-catalyzed steps, including sequential oligonucleotide adapter ligations to the 3′ and 5′ end...

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Αποθηκεύτηκε σε:
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Κύριοι συγγραφείς: Hafner, Markus, Renwick, Neil, Brown, Miguel, Mihailović, Aleksandra, Holoch, Daniel, Lin, Carolina, Pena, John T.G., Nusbaum, Jeffrey D., Morozov, Pavel, Ludwig, Janos, Ojo, Tolulope, Luo, Shujun, Schroth, Gary, Tuschl, Thomas
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: Cold Spring Harbor Laboratory Press 2011
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Article
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3162335/
https://ncbi.nlm.nih.gov/pubmed/21775473
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2799511
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