miRNA in situ hybridization in mammalian tissues fixed with formaldehyde and EDC

MicroRNAs (miRNAs) are small regulatory RNAs with many biological functions and disease associations. We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in significant miRNA loss from mouse tissue sections, which can be prevented by fixation with 1–ethyl–3–(3...

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Detalhes bibliográficos
Main Authors: Pena, John T. G., Sohn-Lee, Cherin, Rouhanifard, Sara H., Ludwig, Janos, Hafner, Markus, Mihailovic, Aleksandra, Lim, Cindy, Holoch, Daniel, Berninger, Philipp, Zavolan, Mihaela, Tuschl, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2838186/
https://ncbi.nlm.nih.gov/pubmed/19137005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1294
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