Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis. In...

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Detalles Bibliográficos

Publicado en:	Proc Natl Acad Sci U S A
Autores principales:	Bryan, P, Pantoliano, M W, Quill, S G, Hsiao, H Y, Poulos, T
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	National Academy of Sciences 1986
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520553/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.11.3743
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