Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis...

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Bibliographic Details
Main Authors: Bryan, P, Pantoliano, M W, Quill, S G, Hsiao, H Y, Poulos, T
Format: Artigo
Language:Inglês
Published: 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC323599/
https://ncbi.nlm.nih.gov/pubmed/3520553
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