Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis. In...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Bryan, P, Pantoliano, M W, Quill, S G, Hsiao, H Y, Poulos, T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1986
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520553/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.11.3743
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