Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis. In...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Bryan, P, Pantoliano, M W, Quill, S G, Hsiao, H Y, Poulos, T
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1986
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520553/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.11.3743
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