Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.
Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis. In...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520553/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.11.3743 |
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