Site-directed mutagenesis and the role of the oxyanion hole in subtilisin.

Oligonucleotide-directed mutagenesis was used to investigate the nature of transition state stabilization in the catalytic mechanism of the serine protease, subtilisin BPN'. The gene for this extracellular enzyme from Bacillus amyloliquefaciens has been cloned and expressed in Bacillus subtilis. In...

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Bibliografische gegevens

Gepubliceerd in:	Proc Natl Acad Sci U S A
Hoofdauteurs:	Bryan, P, Pantoliano, M W, Quill, S G, Hsiao, H Y, Poulos, T
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	National Academy of Sciences 1986
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC323599/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3520553/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.11.3743
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