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Influence of Heme-Thiolate in Shaping the Catalytic Properties of a Bacterial Nitric-oxide Synthase
Nitric-oxide synthases (NOS) are heme-thiolate enzymes that generate nitric oxide (NO) from l-arginine. Mammalian and bacterial NOSs contain a conserved tryptophan (Trp) that hydrogen bonds with the heme-thiolate ligand. We mutated Trp(66) to His and Phe (W66H, W66F) in B. subtilis NOS to investigat...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Biochemistry and Molecular
Biology
2011
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3234747/ https://ncbi.nlm.nih.gov/pubmed/21921039 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.286351 |
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