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Identification of endogenous ligands bound to bacterially expressed human and E. coli dihydrofolate reductase by 2D NMR

Dihydrofolate reductase (DHFR) is a well-studied drug target and a paradigm for understanding enzyme catalysis. Preparation of pure DHFR samples, in defined ligand-bound states, is a prerequisite for in vitro studies and drug discovery efforts. We use NMR spectroscopy to monitor ligand content of hu...

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Detaylı Bibliyografya
Asıl Yazarlar: Bhabha, Gira, Tuttle, Lisa, Martinez-Yamout, Maria, Wright, Peter E.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2011
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3215841/
https://ncbi.nlm.nih.gov/pubmed/22024482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2011.10.014
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