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Identification of endogenous ligands bound to bacterially expressed human and E. coli dihydrofolate reductase by 2D NMR
Dihydrofolate reductase (DHFR) is a well-studied drug target and a paradigm for understanding enzyme catalysis. Preparation of pure DHFR samples, in defined ligand-bound states, is a prerequisite for in vitro studies and drug discovery efforts. We use NMR spectroscopy to monitor ligand content of hu...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2011
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3215841/ https://ncbi.nlm.nih.gov/pubmed/22024482 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.febslet.2011.10.014 |
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