Application of microdroplet PCR for large-scale targeted bisulfite sequencing

Cytosine methylation of DNA CpG dinucleotides in gene promoters is an epigenetic modification that regulates gene transcription. While many methods exist to interrogate methylation states, few current methods offer large-scale, targeted, single CpG resolution. We report an approach combining bisulfi...

Full description

Saved in:
Bibliographic Details
Main Authors: Komori, H. Kiyomi, LaMere, Sarah A., Torkamani, Ali, Hart, G. Traver, Kotsopoulos, Steve, Warner, Jason, Samuels, Michael L., Olson, Jeff, Head, Steven R., Ordoukhanian, Phillip, Lee, Pauline L., Link, Darren R., Salomon, Daniel R.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2011
Subjects:
Method
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3202290/
https://ncbi.nlm.nih.gov/pubmed/21757609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.116863.110
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items