Pipeline for Large-Scale Microdroplet Bisulfite PCR-Based Sequencing Allows the Tracking of Hepitype Evolution in Tumors

Cytosine methylation provides an epigenetic level of cellular plasticity that is important for development, differentiation and cancerogenesis. We adopted microdroplet PCR to bisulfite treated target DNA in combination with second generation sequencing to simultaneously assess DNA sequence and methy...

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Detalhes bibliográficos
Main Authors: Herrmann, Alexander, Haake, Andrea, Ammerpohl, Ole, Martin-Guerrero, Idoia, Szafranski, Karol, Stemshorn, Kathryn, Nothnagel, Michael, Kotsopoulos, Steve K., Richter, Julia, Warner, Jason, Olson, Jeff, Link, Darren R., Schreiber, Stefan, Krawczak, Michael, Platzer, Matthias, Nürnberg, Peter, Siebert, Reiner, Hampe, Jochen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3130030/
https://ncbi.nlm.nih.gov/pubmed/21750708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0021332
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