Application of microdroplet PCR for large-scale targeted bisulfite sequencing

Cytosine methylation of DNA CpG dinucleotides in gene promoters is an epigenetic modification that regulates gene transcription. While many methods exist to interrogate methylation states, few current methods offer large-scale, targeted, single CpG resolution. We report an approach combining bisulfi...

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Detalhes bibliográficos
Main Authors: Komori, H. Kiyomi, LaMere, Sarah A., Torkamani, Ali, Hart, G. Traver, Kotsopoulos, Steve, Warner, Jason, Samuels, Michael L., Olson, Jeff, Head, Steven R., Ordoukhanian, Phillip, Lee, Pauline L., Link, Darren R., Salomon, Daniel R.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2011
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3202290/
https://ncbi.nlm.nih.gov/pubmed/21757609
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.116863.110
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