Microdroplet-based PCR amplification for large scale targeted sequencing

Targeted sequencing of specific loci of the human genome is a promising approach for maximizing the efficiency of second-generation sequencing technologies for population-based studies of genetic variation. Here we describe microdroplet PCR, which performs 1.5 million separate amplifications in para...

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Detalhes bibliográficos
Main Authors: Tewhey, Ryan, Warner, Jason, Nakano, Masakazu, Libby, Brian, Medkova, Martina, David, Patricia, Kotsopoulos, Steve, Samuels, Michael, Hutchison, J. Brian, Larson, Jonathan W., Topol, Eric J., Weiner, Michael P., Harismendy, Olivier, Olson, Jeff, Link, Darren R., Frazer, Kelly A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2779736/
https://ncbi.nlm.nih.gov/pubmed/19881494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1583
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