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Site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed DNA polymerization.
We have utilized infidelity of DNA synthesis as a basis for site-directed mutagenesis. Both an endonuclease restriction fragment and a synthetic oligonucleotide were used as primers. DNA polymerase from bacteriophage T4 was used to elongate primer termini to a position immediately adjacent to two di...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1984
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC320100/ https://ncbi.nlm.nih.gov/pubmed/6089123 |
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