Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry

Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transf...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Miyashita, Masahiro, Presley, Jack M., Buchholz, Bruce A., Lam, Kit S., Lee, Young Moo, Vogel, John S., Hammock, Bruce D.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2001
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11287636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.071047998
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