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Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry

Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transf...

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Detaylı Bibliyografya
Yayımlandı:Proc Natl Acad Sci U S A
Asıl Yazarlar: Miyashita, Masahiro, Presley, Jack M., Buchholz, Bruce A., Lam, Kit S., Lee, Young Moo, Vogel, John S., Hammock, Bruce D.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: National Academy of Sciences 2001
Konular:
Online Erişim:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31847/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11287636/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.071047998
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