Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry
Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transf...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2001
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11287636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.071047998 |
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