Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry

Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transf...

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Argitaratua izan da:	Proc Natl Acad Sci U S A
Egile Nagusiak:	Miyashita, Masahiro, Presley, Jack M., Buchholz, Bruce A., Lam, Kit S., Lee, Young Moo, Vogel, John S., Hammock, Bruce D.
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	National Academy of Sciences 2001
Gaiak:	Biological Sciences
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11287636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.071047998
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