Attomole level protein sequencing by Edman degradation coupled with accelerator mass spectrometry

Edman degradation remains the primary method for determining the sequence of proteins. In this study, accelerator mass spectrometry was used to determine the N-terminal sequence of glutathione S-transferase at the attomole level with zeptomole precision using a tracer of (14)C. The transgenic transf...

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Bibliografiske detaljer

Udgivet i:	Proc Natl Acad Sci U S A
Principais autores:	Miyashita, Masahiro, Presley, Jack M., Buchholz, Bruce A., Lam, Kit S., Lee, Young Moo, Vogel, John S., Hammock, Bruce D.
Format:	Artigo
Sprog:	Inglês
Udgivet:	National Academy of Sciences 2001
Fag:	Biological Sciences
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC31847/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11287636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.071047998
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