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Structure and dynamics of a conformationally constrained nitroxide side chain and applications in EPR spectroscopy

A disulfide-linked nitroxide side chain (R1) is the most widely used spin label for determining protein topology, mapping structural changes, and characterizing nanosecond backbone motions by site-directed spin labeling. Although the internal motion of R1 and the number of preferred rotamers are lim...

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Bibliografiska uppgifter
Huvudupphovsmän: Fleissner, Mark R., Bridges, Michael D., Brooks, Evan K., Cascio, Duilio, Kálai, Tamás, Hideg, Kálmán, Hubbell, Wayne L.
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2011
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3182725/
https://ncbi.nlm.nih.gov/pubmed/21911399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1111420108
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