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Structural determinants of nitroxide motion in spin-labeled proteins: Tertiary contact and solvent-inaccessible sites in helix G of T4 lysozyme

A nitroxide side chain (R1) has been substituted at single sites along a helix–turn–helix motif in T4 lysozyme (residues 114–135). Together with previously published data, the new sites reported complete a continuous scan through the motif. Mutants with R1 at sites 115 and 118 were selected for crys...

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Detalhes bibliográficos
Main Authors: Guo, Zhefeng, Cascio, Duilio, Hideg, Kálmán, Kálái, Támás, Hubbell, Wayne L.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2206656/
https://ncbi.nlm.nih.gov/pubmed/17473014
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.062739107
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