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Unphosphorylatable mutants of Cdc6 disrupt its nuclear export but still support DNA replication once per cell cycle

Cdc6 is essential for eukaryotic DNA replication. We have mutated highly conserved CDK phosphorylation sites in Cdc6. Contrary to their reported phenotypes in human cells, unphosphorylatable ΔCDK mutants fully support DNA replication in Xenopus eggs. WtCdc6 is actively exported from the nucleus, whi...

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Detalhes bibliográficos
Main Authors: Pelizon, Cristina, Madine, Mark A., Romanowski, Piotr, Laskey, Ronald A.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC316983/
https://ncbi.nlm.nih.gov/pubmed/11018019
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