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Lys98 Substitution in Human AP Endonuclease 1 Affects the Kinetic Mechanism of Enzyme Action in Base Excision and Nucleotide Incision Repair Pathways

Human apurinic/apyrimidinic endonuclease 1 (APE1) is a key enzyme in the base excision repair (BER) and nucleotide incision repair (NIR) pathways. We recently analyzed the conformational dynamics and kinetic mechanism of wild-type (wt) protein, in a stopped-flow fluorescence study. In this study, we...

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Bibliografische gegevens
Hoofdauteurs: Timofeyeva, Nadezhda A., Koval, Vladimir V., Ishchenko, Alexander A., Saparbaev, Murat K., Fedorova, Olga S.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2011
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3164677/
https://ncbi.nlm.nih.gov/pubmed/21912662
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0024063
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