Neutralizing Mutations of Carboxylates That Bind Metal 2 in T5 Flap Endonuclease Result in an Enzyme That Still Requires Two Metal Ions

Flap endonucleases (FENs) are divalent metal ion-dependent phosphodiesterases. Metallonucleases are often assigned a “two-metal ion mechanism” where both metals contact the scissile phosphate diester. The spacing of the two metal ions observed in T5FEN structures appears to preclude this mechanism....

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Main Authors: Tomlinson, Christopher G., Syson, Karl, Sengerová, Blanka, Atack, John M., Sayers, Jon R., Swanson, Linda, Tainer, John A., Williams, Nicholas H., Grasby, Jane A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2011
Teme:
Enzymology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3162448/
https://ncbi.nlm.nih.gov/pubmed/21734257
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.230391
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