Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel (34)S labeling procedure

BACKGROUND: The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intr...

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Библиографические подробности
Главные авторы: Pfeffer, Martin, Maurer, Michael, Köllensperger, Gunda, Hann, Stephan, Graf, Alexandra B, Mattanovich, Diethard
Формат: Artigo
Язык:Inglês
Опубликовано: BioMed Central 2011
Предметы:
Research
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3147017/
https://ncbi.nlm.nih.gov/pubmed/21703020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-47
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