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Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel (34)S labeling procedure

BACKGROUND: The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intr...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Pfeffer, Martin, Maurer, Michael, Köllensperger, Gunda, Hann, Stephan, Graf, Alexandra B, Mattanovich, Diethard
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2011
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3147017/
https://ncbi.nlm.nih.gov/pubmed/21703020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-47
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