An approach to genomewide screens of expressed small interfering RNAs in mammalian cells

To facilitate the construction of large genomewide libraries of small interfering RNAs (siRNAs), we have developed a dual promoter system (pDual) in which a synthetic DNA encoding a gene-specific siRNA sequence is inserted between two different opposing polymerase III promoters, the mouse U6 and hum...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Zheng, Lianxing, Liu, Jun, Batalov, Sergei, Zhou, Demin, Orth, Anthony, Ding, Sheng, Schultz, Peter G.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2003
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC314151/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14688408/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.2136685100
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