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An approach to genomewide screens of expressed small interfering RNAs in mammalian cells
To facilitate the construction of large genomewide libraries of small interfering RNAs (siRNAs), we have developed a dual promoter system (pDual) in which a synthetic DNA encoding a gene-specific siRNA sequence is inserted between two different opposing polymerase III promoters, the mouse U6 and hum...
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| Autors principals: | , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2004
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC314151/ https://ncbi.nlm.nih.gov/pubmed/14688408 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2136685100 |
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