An approach to genomewide screens of expressed small interfering RNAs in mammalian cells

To facilitate the construction of large genomewide libraries of small interfering RNAs (siRNAs), we have developed a dual promoter system (pDual) in which a synthetic DNA encoding a gene-specific siRNA sequence is inserted between two different opposing polymerase III promoters, the mouse U6 and hum...

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Bibliographic Details
Main Authors: Zheng, Lianxing, Liu, Jun, Batalov, Sergei, Zhou, Demin, Orth, Anthony, Ding, Sheng, Schultz, Peter G.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2004
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC314151/
https://ncbi.nlm.nih.gov/pubmed/14688408
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2136685100
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