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Cloning and expression of the HpaI restriction-modification genes.

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 31...

詳細記述

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書誌詳細
主要な著者: Ito, H, Shimato, H, Sadaoka, A, Kotani, H, Kimizuka, F, Kato, I
フォーマット: Artigo
言語:Inglês
出版事項: 1992
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC312008/
https://ncbi.nlm.nih.gov/pubmed/1542567
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