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Control of partial digestion combining the enzymes dam methylase and MboI.

A method is described which allows the preparation of reproducible partial digests without previous establishment of the incubation conditions. It is based on a combined application of dam methylase and the restriction endonuclease MboI, both recognizing the sequence 5'-GATC-3' but MboI un...

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Detalhes bibliográficos
Main Authors: Hoheisel, J D, Nizetic, D, Lehrach, H
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC335198/
https://ncbi.nlm.nih.gov/pubmed/2602137
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