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Control of partial digestion combining the enzymes dam methylase and MboI.
A method is described which allows the preparation of reproducible partial digests without previous establishment of the incubation conditions. It is based on a combined application of dam methylase and the restriction endonuclease MboI, both recognizing the sequence 5'-GATC-3' but MboI un...
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| Main Authors: | , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1989
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC335198/ https://ncbi.nlm.nih.gov/pubmed/2602137 |
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