Control of partial digestion combining the enzymes dam methylase and MboI.
A method is described which allows the preparation of reproducible partial digests without previous establishment of the incubation conditions. It is based on a combined application of dam methylase and the restriction endonuclease MboI, both recognizing the sequence 5'-GATC-3' but MboI unable to cu...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1989
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC335198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2602137/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.23.9571 |
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