Cloning and expression of the HpaI restriction-modification genes.

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 31...

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Detalles Bibliográficos

Publicado en:	Nucleic Acids Res
Principais autores:	Ito, H, Shimato, H, Sadaoka, A, Kotani, H, Kimizuka, F, Kato, I
Formato:	Artigo
Idioma:	Inglês
Publicado:	Oxford University Press 1992
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312008/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1542567/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.4.705
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