Cloning and expression of the HpaI restriction-modification genes.

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 31...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Ito, H, Shimato, H, Sadaoka, A, Kotani, H, Kimizuka, F, Kato, I
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1992
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312008/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1542567/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.4.705
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