Cloning and expression of the HpaI restriction-modification genes.

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 31...

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Bibliografiska uppgifter
Huvudupphovsmän: Ito, H, Shimato, H, Sadaoka, A, Kotani, H, Kimizuka, F, Kato, I
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1992
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC312008/
https://ncbi.nlm.nih.gov/pubmed/1542567
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