Cloning and expression of the HpaI restriction-modification genes.

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 31...

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Bibliografiske detaljer
Udgivet i:Nucleic Acids Res
Main Authors: Ito, H, Shimato, H, Sadaoka, A, Kotani, H, Kimizuka, F, Kato, I
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1992
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312008/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1542567/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.4.705
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