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Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the l...

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Bibliografiske detaljer
Main Authors: de Vroom, E, Fidder, A, Marugg, J E, van der Marel, G A, van Boom, J H
Format: Artigo
Sprog:Inglês
Udgivet: 1986
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC311598/
https://ncbi.nlm.nih.gov/pubmed/2426660
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