Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.
Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the l...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Principais autores: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1986
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| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311598/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2426660/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.14.5885 |
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