Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.

Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the l...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	de Vroom, E, Fidder, A, Marugg, J E, van der Marel, G A, van Boom, J H
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 1986
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311598/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2426660/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/14.14.5885
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