Genome-Scale Cloning and Expression of Individual Open Reading Frames Using Topoisomerase I-Mediated Ligation

The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Va...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Genome Res
Autori principali: Heyman, John A., Cornthwaite, Jeremiah, Foncerrada, Luis, Gilmore, Jeremiah R., Gontang, Erin, Hartman, Kristen J., Hernandez, Cathy L., Hood, Rhiannon, Hull, Heather M., Lee, Wai-Yee, Marcil, Robert, Marsh, Ed J., Mudd, Kevin M., Patino, Mario J., Purcell, Thomas J., Rowland, Jon J., Sindici, Michelle L., Hoeffler, James P.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 1999
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310734/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10207160/
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