Genome-Scale Cloning and Expression of Individual Open Reading Frames Using Topoisomerase I-Mediated Ligation

The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Va...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Heyman, John A., Cornthwaite, Jeremiah, Foncerrada, Luis, Gilmore, Jeremiah R., Gontang, Erin, Hartman, Kristen J., Hernandez, Cathy L., Hood, Rhiannon, Hull, Heather M., Lee, Wai-Yee, Marcil, Robert, Marsh, Ed J., Mudd, Kevin M., Patino, Mario J., Purcell, Thomas J., Rowland, Jon J., Sindici, Michelle L., Hoeffler, James P.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Cold Spring Harbor Laboratory Press 1999
Gaiak:
Methods
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC310734/
https://ncbi.nlm.nih.gov/pubmed/10207160
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