Genome-Scale Cloning and Expression of Individual Open Reading Frames Using Topoisomerase I-Mediated Ligation

The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Va...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Heyman, John A., Cornthwaite, Jeremiah, Foncerrada, Luis, Gilmore, Jeremiah R., Gontang, Erin, Hartman, Kristen J., Hernandez, Cathy L., Hood, Rhiannon, Hull, Heather M., Lee, Wai-Yee, Marcil, Robert, Marsh, Ed J., Mudd, Kevin M., Patino, Mario J., Purcell, Thomas J., Rowland, Jon J., Sindici, Michelle L., Hoeffler, James P.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 1999
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC310734/
https://ncbi.nlm.nih.gov/pubmed/10207160
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados