Genome-Scale Cloning and Expression of Individual Open Reading Frames Using Topoisomerase I-Mediated Ligation

The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the corresponding ends. We have used the properties of Va...

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Autors principals: Heyman, John A., Cornthwaite, Jeremiah, Foncerrada, Luis, Gilmore, Jeremiah R., Gontang, Erin, Hartman, Kristen J., Hernandez, Cathy L., Hood, Rhiannon, Hull, Heather M., Lee, Wai-Yee, Marcil, Robert, Marsh, Ed J., Mudd, Kevin M., Patino, Mario J., Purcell, Thomas J., Rowland, Jon J., Sindici, Michelle L., Hoeffler, James P.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1999
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC310734/
https://ncbi.nlm.nih.gov/pubmed/10207160
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