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DNA strand transfer catalyzed by vaccinia topoisomerase: ligation of DNAs containing a 3′ mononucleotide overhang

The specificity of vaccinia topoisomerase for transesterification to DNA at the sequence 5′-CCCTT and its versatility in strand transfer have illuminated the recombinogenic properties of type IB topoisomerases and spawned topoisomerase-based strategies for DNA cloning. Here we characterize a pathway...

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Bibliografiske detaljer
Main Authors: Cheng, Chonghui, Shuman, Stewart
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2000
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC103307/
https://ncbi.nlm.nih.gov/pubmed/10756188
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