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In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins.

We have used purified RSF1010 mobilization proteins to reproduce in vitro a strand-specific nicking at the plasmid origin of transfer, oriT. In the presence of Mg2+, the proteins MobA (78-kDa form of RSF1010 DNA primase), MobB, and MobC and supercoiled or linear duplex oriT DNA form large amounts of...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Scherzinger, E, Lurz, R, Otto, S, Dobrinski, B
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1992
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC310323/
https://ncbi.nlm.nih.gov/pubmed/1738602
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