In vitro cleavage of double- and single-stranded DNA by plasmid RSF1010-encoded mobilization proteins.
We have used purified RSF1010 mobilization proteins to reproduce in vitro a strand-specific nicking at the plasmid origin of transfer, oriT. In the presence of Mg2+, the proteins MobA (78-kDa form of RSF1010 DNA primase), MobB, and MobC and supercoiled or linear duplex oriT DNA form large amounts of...
Guardat en:
| Publicat a: | Nucleic Acids Res |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1992
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310323/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1738602/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.1.41 |
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